Tift County High School

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DNA Extraction

Students in Mrs. Richard’s Applications of Biotechnology class participated in a DNA Extraction Lab. They used an enzyme called protease along with a lysis buffer, salt, and a hot water bath to break open their own cheek cells and collect the DNA. After the extraction was complete, they transferred their DNA from the test tube to a vial on a necklace. The blue dye makes the DNA easier to see. This lab followed a unit on Cell Membrane Manipulation which included discussions on electroporesis (using electricity to open the cell membrane to allow DNA or medicine in), microprojectile bombardment ("Gene Gun" shoots DNA into a tissue), and ionic stress (Uses chemicals to open the cell membrane). The unit also included a section on applications of these three procedures.

Pictured are Claudia Turner and Rachel Perez.